PARG Antibody / Poly(ADP-ribose) glycohydrolase, 100 ug

PARG antibody detects Poly(ADP-ribose) glycohydrolase, encoded by the PARG gene on chromosome 10q11.23. PARG antibody is widely used in DNA damage response, chromatin remodeling, and cancer biology research. PARG is the main enzyme responsible for degrading poly(ADP-ribose) chains, which are synthesized by PARP family enzymes in response to DNA breaks and stress signals. By hydrolyzing poly(ADP-ribose), PARG resets chromatin state and restores protein activity following PARP-mediated modifications.

Structurally, PARG is a ~110 kDa enzyme with a macrodomain-containing catalytic region. It cleaves glycosidic ribose-ribose bonds in poly(ADP-ribose) chains, generating free ADP-ribose units. PARG localizes predominantly to the nucleus but is also found in cytoplasm and mitochondria, reflecting diverse roles in cellular stress responses. Alternative isoforms, including shorter forms, may localize differently and regulate specific processes.

Functionally, PARG counteracts PARP activity to maintain balance in ADP-ribosylation signaling. After DNA damage, PARP1 rapidly polymerizes ADP-ribose chains that recruit DNA repair factors. PARG then degrades these chains to terminate signaling, recycle NAD+, and permit chromatin resetting. Dysregulation of PARG disrupts repair efficiency and genomic stability. Researchers use PARG antibody to study DNA repair, chromatin biology, and PARP inhibitor responses.

Clinically, PARG is relevant in cancer therapy because PARP inhibitors exploit defective homologous recombination repair in tumors. PARG inhibition further sensitizes cancer cells to DNA damage, making it a therapeutic target. PARG dysregulation has also been implicated in neurodegeneration, where abnormal ADP-ribose metabolism contributes to cell death. NSJ Bioreagents supplies PARG antibody for research in DNA repair, cancer biology, and therapeutic development.

Experimentally, PARG antibody is applied in western blotting to detect the ~110 kDa protein, in immunohistochemistry to study nuclear expression, and in enzymatic assays to monitor ADP-ribose metabolism. Co-immunoprecipitation with PARG antibody identifies complexes with PARP and DNA repair proteins.