NUP43 Antibody / Nucleoporin 43, 100 ug

NUP43 antibody detects Nuclear pore complex protein Nup43, encoded by the NUP43 gene on chromosome 6p21.33. NUP43 antibody is widely used in studies of nucleocytoplasmic transport, nuclear pore structure, and gene regulation. NUP43 is one of the small, conserved subunits of the nuclear pore complex (NPC), a massive macromolecular assembly that regulates bidirectional transport of RNA, proteins, and signaling molecules across the nuclear envelope. By interacting with scaffold nucleoporins, NUP43 helps stabilize the Y-complex, a structural subassembly of the NPC.

Structurally, NUP43 is a ~43 kDa protein with a WD40 beta-propeller domain that mediates protein-protein interactions. It interacts with NUP107, NUP133, and other Y-complex components to form a stable subcomplex essential for pore assembly. NUP43 localization is restricted to the nuclear envelope, consistent with its structural role in NPC architecture. Its highly conserved nature across species emphasizes its importance in nuclear transport machinery.

Functionally, NUP43 supports NPC assembly, nuclear import and export, and chromatin organization. During mitosis, the Y-complex including NUP43 contributes to reformation of the nuclear envelope after breakdown. Loss of NUP43 destabilizes the Y-complex, impairing nuclear transport and genome organization. Researchers use NUP43 antibody to study nuclear pore biology, gene expression regulation, and nuclear architecture.

Clinically, NUP43 has been linked to cancer and developmental disorders. Altered expression and mutations in nucleoporins, including NUP43, are associated with leukemias and solid tumors, where transport dysregulation promotes abnormal signaling. Variants in NUP43 have also been implicated in neurodevelopmental disorders due to impaired nuclear-cytoplasmic communication. NSJ Bioreagents provides NUP43 antibody for use in nuclear transport, cancer, and developmental biology research.

Experimentally, NUP43 antibody is used in western blotting to detect the ~43 kDa protein, in immunofluorescence to highlight nuclear envelope localization, and in immunohistochemistry to analyze expression in tissue sections. Co-immunoprecipitation with NUP43 antibody identifies interaction partners within the NPC Y-complex.