PLS3 Antibody / Plastin 3, 100 ug

PLS3 antibody detects Plastin-3, encoded by the PLS3 gene on chromosome Xq23. PLS3 antibody is widely used in research on actin dynamics, cytoskeletal regulation, and bone disease. Plastin-3 belongs to the plastin family of actin-bundling proteins, which regulate the organization of actin filaments in diverse cellular processes including adhesion, migration, endocytosis, and vesicle trafficking. PLS3 is expressed in many tissues, with enrichment in intestine, kidney, and bone, and is critical for cytoskeletal integrity and mechanosensing.

Structurally, Plastin-3 is a ~70 kDa protein containing two actin-binding domains and a regulatory calcium-binding EF-hand motif. The dual actin-binding domains enable crosslinking of actin filaments into bundles, while the EF-hand motif confers regulation by calcium signaling. Isoforms generated by alternative splicing provide tissue-specific modulation of activity.

Functionally, PLS3 stabilizes actin structures required for cell adhesion, vesicle trafficking, and mechanotransduction. In osteocytes, PLS3 contributes to sensing mechanical load and bone remodeling. In epithelial cells, it supports brush border formation and endocytic processes. Loss of function impairs cytoskeletal stability, leading to cellular fragility and disease phenotypes. Researchers use PLS3 antibody to study actin remodeling, mechanobiology, and cytoskeletal disorders.

Clinically, mutations in PLS3 are associated with X-linked osteoporosis, a rare bone fragility disorder characterized by low bone mineral density and fracture susceptibility. Variants in PLS3 impair actin bundling in osteocytes, disrupting mechanosensing and bone homeostasis. PLS3 has also been implicated in cancer, where altered expression influences invasion and metastasis. NSJ Bioreagents supplies PLS3 antibody for use in bone biology, cytoskeletal research, and oncology.

Experimentally, PLS3 antibody is applied in western blotting to detect the ~70 kDa protein, in immunofluorescence to visualize actin bundles, and in immunohistochemistry to study tissue distribution. Co-immunoprecipitation with PLS3 antibody identifies actin-binding partners and regulatory proteins. Functional studies using PLS3 antibody help link cytoskeletal organization with mechanical and signaling pathways.