MYH14 Antibody / Myosin 14, 100 ug

MYH14 antibody detects Myosin-14, encoded by the MYH14 gene on chromosome 19q13.33. MYH14 antibody is used in studies of cytoskeletal regulation, hearing, and cellular motility. Myosin-14 belongs to the class II non-muscle myosins, functioning as an actin-based motor protein. It regulates actin filament organization, adhesion, and vesicle trafficking. Expression is widespread, with high levels in inner ear hair cells, smooth muscle, and neuronal tissue, where it supports specialized mechanical and sensory functions.

Structurally, Myosin-14 is a large protein containing an N-terminal motor domain with ATPase activity, a neck region with IQ motifs that bind light chains, and a coiled-coil tail that mediates dimerization and filament assembly. Its motor domain hydrolyzes ATP to generate force for actin filament sliding, supporting contractility and cytoskeletal rearrangements. Isoforms derived from alternative splicing confer tissue-specific functions.

Functionally, Myosin-14 regulates actin cytoskeleton dynamics, contributing to cell migration, adhesion, and intracellular transport. In neurons, it supports axon guidance and synaptic function. In the inner ear, Myosin-14 is essential for mechanotransduction and hearing. Knockout or mutation of MYH14 disrupts hair cell function, leading to progressive hearing loss. Researchers use MYH14 antibody to investigate cytoskeletal biology, neuronal function, and auditory physiology.

Clinically, MYH14 mutations are associated with autosomal dominant non-syndromic hearing loss (DFNA4A). Mutations disrupt motor activity or actin binding, impairing hair cell function. Myosin-14 variants are also linked to peripheral neuropathy and craniofacial developmental disorders. Altered expression of MYH14 has been observed in cancers, where cytoskeletal regulation influences invasion and metastasis. NSJ Bioreagents offers MYH14 antibody as a tool for studies of cytoskeletal regulation, auditory biology, and disease mechanisms.

Experimentally, MYH14 antibody is used in western blotting to detect the ~230 kDa protein, in immunofluorescence microscopy to visualize actin-associated localization, and in immunohistochemistry to study hair cell and neuronal expression. Co-immunoprecipitation with MYH14 antibody isolates complexes of actin, myosin light chains, and regulatory proteins.