PNKP Antibody / Polynucleotide kinase 3 phosphatase, 100 ul

PNKP antibody detects polynucleotide kinase 3'-phosphatase, a DNA repair enzyme with dual kinase and phosphatase activities. PNKP processes DNA ends by phosphorylating 5' termini and dephosphorylating 3' termini, preparing them for ligation during single-strand and double-strand break repair. This enzyme functions in multiple repair pathways, including base excision repair and non-homologous end joining, ensuring genomic stability after DNA damage.

Research using PNKP antibody has revealed the protein's significance in neurodegenerative diseases and cancer. Mutations in PNKP cause neurological disorders such as microcephaly with seizures and developmental delay (MCSZ), where defective DNA repair leads to progressive neuronal loss. In oncology, PNKP is upregulated in certain tumors, where it contributes to resistance against radiation and chemotherapy by repairing therapy-induced DNA damage. Targeting PNKP is therefore a promising therapeutic strategy to sensitize tumors to genotoxic treatments.

PNKP also plays a role in maintaining mitochondrial DNA integrity, protecting cells from oxidative stress. Its activity is essential for neuronal survival and for preventing age-related genomic instability. Understanding PNKP regulation provides insight into how cells balance repair efficiency with the risk of mutagenesis.

Antibodies against PNKP are validated for use in western blot, immunofluorescence, immunoprecipitation, and immunohistochemistry. These tools allow researchers to track DNA repair activity, analyze protein localization in nuclei and mitochondria, and explore mechanisms of therapy resistance. Clone-based antibodies ensure reproducible results across studies.

NSJ Bioreagents supplies this PNKP antibody to support research in DNA repair, neurobiology, and cancer therapeutics.