NSJ Bioreagents
SKU:F48111-0.08ML
Cyclin D1 Antibody / CCND1, 0.08ml
Cyclin D1 Antibody / CCND1, 0.08ml
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CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. [UniProt]
Specifications
| Catalog No | F48111-0.08ML |
|---|---|
| Family | Primary antibody |
| Qty | 0.08ml |
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human, Mouse |
| Predicted Species Reactivity | Bovine |
| Application | WB, IHC, IF, ELISA |
| Application Details | Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50 |
| Application Note | Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Status | Available |
| PDF Link | Product Data Sheet (PDF) |
| Limitation | This Cyclin D1 antibody is available for research use only. |
| Uniprot # | P24385 |
| Purity | Antigen affinity |
| Immunogen | A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody. |
| Storage | Aliquot the Cyclin D1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Title | Cyclin D1 Antibody / CCND1 |
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