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NSJ Bioreagents
SKU:F47834-0.08ML
NES Antibody / Nestin, 0.08ml
NES Antibody / Nestin, 0.08ml
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Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Specifications
| Catalog No | F47834-0.08ML |
|---|---|
| Family | Primary antibody |
| Qty | 0.08ml |
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human |
| Application | WB, IHC, IF, FACS, ELISA |
| Application Details | Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50 |
| Application Note | Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Status | Available |
| PDF Link | Product Data Sheet (PDF) |
| Limitation | This Nestin antibody is available for research use only. |
| Uniprot # | P48681 |
| Purity | Antigen affinity |
| Immunogen | A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody. |
| Storage | Aliquot the Nestin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Localization | Cytoplasmic, membranous |
| Title | NES Antibody / Nestin |
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