• Western blot testing of human MCF7 cell lysate with CYBB antibody. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.
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NSJ Bioreagents

RQ5084

CYBB Antibody / NOX2 / gp91phox

CYBB Antibody / NOX2 / gp91phox

$449.00 USD
$449.00 USD
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Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. [UniProt]

Specifications

Family Primary antibody
Formulation Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Format Purified
Clone GOH-3
Host Animal Rabbit
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Species Reactivity Human
Application WB
Application Details Western blot: 1:1000-1:2000
Application Note Optimal dilution of the CYBB antibody should be determined by the researcher.
Immunogen A synthetic peptide specific to human Nox2 / CYBB was used as the immunogen for the CYBB antibody.
Purity Affinity purified
Storage Store the CYBB antibody at -20oC.
Limitation This CYBB antibody is available for research use only.
Uniprot # P04839
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cybb-antibody-nox2-gp91phox-goh-3-rq5084
Title CYBB Antibody / NOX2 / gp91phox
Description Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. [UniProt]
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