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NSJ Bioreagents

SKU:R30201

Cx43 Antibody / Connexin 43

Cx43 Antibody / Connexin 43

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Connexin 43 (Cx43), also called GAP Junction Protein, alpha, is a member of the connexin gene family and is abundantly expressed in the heart and liver. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human (1), Mouse, Rat
Application WB, IHC-P, IHC-F
Application Details Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
Application Note Titration of the Cx43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.<br><br>1.Suitability for western blot testing of human samples is still being determined.
Localization Cytoplasm, plasma membrane
Immunogen Amino acids 351-367 (HELQPLAIVDQRPSSRA-human) were used as the immunogen for this Cx43 antibody (100% conserved in mouse and rat).
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity Antigen affinity
Storage The lyophilized Cx43 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Cx43 antibody is available for research use only.
Uniprot # P17302
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cx43-antibody-connexin-43-r30201
Title Cx43 Antibody / Connexin 43
Description Connexin 43 (Cx43), also called GAP Junction Protein, alpha, is a member of the connexin gene family and is abundantly expressed in the heart and liver. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
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