• Fluorescent confocal image of human HeLa cell stained with CUX1 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.
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NSJ Bioreagents

F49389-0.08ML

CUX1 Antibody / Homeobox protein cut-like 1

CUX1 Antibody / Homeobox protein cut-like 1

$205.00 USD
$205.00 USD
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CUX1 likely has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). [UniProt]

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, IHC, IF, ELISA
Application Details Western blot: 1:1000-1:2000,IHC (Paraffin): 1:25-1:50,Immunofluorescence: 1:10-1:50
Application Note Titration of the CUX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 1347-1374 from the human protein was used as the immunogen for this CUX1 antibody.
Purity Antigen affinity
Storage Aliquot the CUX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This CUX1 antibody is available for research use only.
Uniprot # P39880
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cux1-antibody-homeobox-protein-cut-like-1-f49389
Title CUX1 Antibody / Homeobox protein cut-like 1
Description CUX1 likely has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). [UniProt]
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