• Western blot testing of human 1) WiDr, 2) Raji, 3) K562, 4) Jurkat and 5) CCRF-CEM cell lysate with CTLA4 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.
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NSJ Bioreagents

F55061-0.05ML

CTLA4 Antibody / Cytotoxic T-lymphocyte protein 4 / CD152

CTLA4 Antibody / Cytotoxic T-lymphocyte protein 4 / CD152

$205.00 USD
$205.00 USD
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CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, IHC-P
Application Details Western blot: 1:1000-1:2000,Immunofluorescence: 1:50-1:100
Application Note Titration of the CTLA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 182-210 from the human protein was used as the immunogen for this CTLA4 antibody.
Purity Antigen affinity
Storage Aliquot the CTLA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This CTLA4 antibody is available for research use only.
Uniprot # P16410
Status Available
PDF Link http://www.nsjbio.com/tds-pdf/ctla4-antibody-cytotoxic-t-lymphocyte-protein-4-cd152-f55061
Title CTLA4 Antibody / Cytotoxic T-lymphocyte protein 4 / CD152
Description CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.
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