• IHC testing of FFPE human lung cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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NSJ Bioreagents

R32782

CPI-17 Antibody / PPP1R14A

CPI-17 Antibody / PPP1R14A

$449.00 USD
$449.00 USD
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Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, FACS, IF
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence: 2-4ug/ml
Application Note Optimal dilution of the CPI-17 antibody should be determined by the researcher.
Localization Cytoplasmic, nuclear
Immunogen Amino acids L30-Q126 from human CPI-17 alpha were used as the immunogen for the CPI-17 antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Purity Antigen affinity
Storage After reconstitution, the CPI-17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This CPI-17 antibody is available for research use only.
Uniprot # Q96A00
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cpi-17-antibody-ppp1r14a-r32782
Title CPI-17 Antibody / PPP1R14A
Description Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
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