• IHC testing of FFPE human spleen tissue with COTL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F55004-0.08ML

COTL1 Antibody / Coactosin-like protein

COTL1 Antibody / Coactosin-like protein

$205.00 USD
$205.00 USD
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COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, FACS, IHC-P
Application Details Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note The stated application concentrations are suggested starting points. Titration of the COTL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic, nuclear
Immunogen A portion of amino acids 115-142 from the human protein was used as the immunogen for the COTL1 antibody.
Purity Antigen affinity purified
Storage Aliquot the COTL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This COTL1 antibody is available for research use only.
Uniprot # Q14019
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cotl1-antibody-coactosin-like-protein-f55004
Title COTL1 Antibody / Coactosin-like protein
Description COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
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