• IHC staining of FFPE human tonsil with COPE antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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NSJ Bioreagents

RQ5522

COPE Antibody

COPE Antibody

$449.00 USD
$449.00 USD
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Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Clone 9B6
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, FACS
Application Details Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Flow cytometry: 1-3ug/million cells
Application Note Optimal dilution of the COPE antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen A human recombinant protein (amino acids E80-A308) was used as the immunogen for the COPE antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Affinity purified
Storage After reconstitution, the COPE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This COPE antibody is available for research use only.
Uniprot # O14579
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cope-antibody-9b6-rq5522
Title COPE Antibody
Description Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
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