• Western blot testing of Connexin 32 antibody and Lane 1: rat heart; 2: (r) heart; 3: (r) skeletal muscle; 4: (r) brain; 5: human MCF-7; 6: (h) HeLa; 7: (h) SMMC-7721; 8: (h) Jurkat; 9: (h) COLO320 lysate. Predicted molecular weight ~32 kDa.
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NSJ Bioreagents

R30436

Connexin 32 Antibody

Connexin 32 Antibody

$449.00 USD
$449.00 USD
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Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P
Application Details Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml
Application Note The stated application concentrations are suggested starting amounts. Titration of the Connexin 32 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cell surface, cytoplasmic
Immunogen An amino acid sequence from the middle region of human Connexin 32/GJB1 (RACARRAQRRSNPPSRK) was used as the immunogen for this Connexin 32 antibody (100% homologous in human, mouse and rat).
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity Antigen affinity
Storage After reconstitution, the Connexin 32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Connexin 32 antibody is available for research use only.
Uniprot # P08034
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/connexin-32-antibody-r30436
Title Connexin 32 Antibody
Description Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.
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