• Immunofluorescent staining of FFPE human placental tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.
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NSJ Bioreagents

R31258

Collagen 1 Antibody

Collagen 1 Antibody

$449.00 USD
$449.00 USD
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Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, ICC, IF
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunocytochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml
Application Note The stated application concentrations are suggested starting amounts. Titration of the Collagen 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic
Immunogen An amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen 1 antibody (100% rat homology).
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity
Storage After reconstitution, the Collagen 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Collagen 1 antibody is available for research use only.
Uniprot # P02452
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/collagen-1-antibody-r31258
Title Collagen 1 Antibody
Description Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
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