• IHC testing of FFPE human lung carcinoma tissue with Clathrin Light Chain A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F55014-0.08ML

Clathrin Light Chain A Antibody / CTLA

Clathrin Light Chain A Antibody / CTLA

$205.00 USD
$205.00 USD
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Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application FACS, WB, IHC-P
Application Details Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note The stated application concentrations are suggested starting points. Titration of the Clathrin Light Chain A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic
Immunogen A portion of amino acids 99-127 from the human protein was used as the immunogen for the Clathrin Light Chain A antibody.
Purity Antigen affinity purified
Storage Aliquot the Clathrin Light Chain A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Clathrin Light Chain A antibody is available for research use only.
Uniprot # P09496
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/clathrin-light-chain-a-antibody-ctla-f55014
Title Clathrin Light Chain A Antibody / CTLA
Description Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.
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