• Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) SW620, 5) MDA-MB-231, 6) rat brain and 7) mouse brain lysate with CKB antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
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NSJ Bioreagents

RQ4327

CKB Antibody / Creatine kinase B

CKB Antibody / Creatine kinase B

$449.00 USD
$449.00 USD
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Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, ELISA
Application Details Western blot: 0.5-1ug/ml, IHC (FFPE): 1-2ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the CKB antibody should be determined by the researcher.
Localization Cytoplasm
Immunogen A recombinant human protein corresponding to amino acids H7-K381 was used as the immunogen for the CKB antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Antigen affinity purified
Storage After reconstitution, the CKB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This CKB antibody is available for research use only.
Uniprot # P12277
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/ckb-antibody-creatine-kinase-b-rq4327
Title CKB Antibody / Creatine kinase B
Description Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
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