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NSJ Bioreagents

SKU:R32638

CHK1 Antibody / Checkpoint kinase 1

CHK1 Antibody / Checkpoint kinase 1

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CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human
Application WB, FACS
Application Details Western blot: 0.5-1ug/ml,FACS: 1-3ug/million cells
Application Note Optimal dilution of the CHK1 antibody should be determined by the researcher.
Immunogen Amino acids M1-Q210 from the human protein were used as the immunogen for the CHK1 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity
Storage After reconstitution, the CHK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This CHK1 antibody is available for research use only.
Uniprot # O14757
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/chk1-antibody-checkpoint-kinase-1-r32638
Title CHK1 Antibody / Checkpoint kinase 1
Description CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.
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