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NSJ Bioreagents
SKU:RQ7726
CHGA Antibody / Chromogranin A
CHGA Antibody / Chromogranin A
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CHGA, also known as CGA or Chromogranin A, is an acidic protein co-stored and co-released with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine).
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Application | WB, IHC-P, FACS, Direct ELISA |
| Application Details | Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml |
| Application Note | Optimal dilution of the CHGA antibody should be determined by the researcher. |
| Localization | Finely granular cytoplasmic |
| Immunogen | E. coli-derived recombinant human protein (amino acids L19-E417) was used as the immunogen for the CHGA antibody. |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| Purity | Antigen affinity purified |
| Storage | After reconstitution, the CHGA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This CHGA antibody is available for research use only. |
| Uniprot # | P10645 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/chga-antibody-chromogranin-a-rq7726 |
| Title | CHGA Antibody / Chromogranin A |
| Description | CHGA, also known as CGA or Chromogranin A, is an acidic protein co-stored and co-released with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). |
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