NSJ Bioreagents
SKU:F54670-0.08ML
CDKN1A Antibody / p21WAF1
CDKN1A Antibody / p21WAF1
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This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human, Mouse |
| Application | WB, IF, IHC-P |
| Application Details | Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25 |
| Application Note | The stated application concentrations are suggested starting points. Titration of the CDKN1A antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Localization | Nuclear, cytoplasmic |
| Immunogen | A portion of amino acids 117-146 from the human protein was used as the immunogen for the CDKN1A antibody. |
| Purity | Antigen affinity purified |
| Storage | Aliquot the CDKN1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This CDKN1A antibody is available for research use only. |
| Uniprot # | P38936 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/cdkn1a-antibody-p21waf1-f54670 |
| Title | CDKN1A Antibody / p21WAF1 |
| Description | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. |
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