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NSJ Bioreagents

SKU:R30624

Cdk5 Antibody

Cdk5 Antibody

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Cyclin-Dependent Kinase 5, is also know as PSSALRE. The p34(CDC2) protein kinase regulates important transitions in the eukaryotic cell cycle. Cdk5 expression is in all human tissues and cell lines tested. The the gene is mapped to chromosome 7q36. Cyclin-dependent kinase 5 is predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau. It is required for proper development of the mammalian central nervous system. To be activated, Cdk5 must associate with its regulatory subunit, p35(CDK5R1). It can phosphorylate DARPP32 at threonine-75, converting it into an inhibitor of PKA.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB
Application Details Western blot: 0.5-1ug/ml
Application Note The stated application concentrations are suggested starting amounts. Titration of the Cdk5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen An amino acid sequence from the C-terminus of human Cdk5 (QRISAEEALQHPYFSDFCPP) was used as the immunogen for this Cdk5 antibody (100% homologous in human, mouse and rat).
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity Antigen affinity
Storage After reconstitution, the Cdk5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Cdk5 antibody is available for research use only.
Uniprot # Q00535
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cdk5-antibody-r30624
Title Cdk5 Antibody
Description Cyclin-Dependent Kinase 5, is also know as PSSALRE. The p34(CDC2) protein kinase regulates important transitions in the eukaryotic cell cycle. Cdk5 expression is in all human tissues and cell lines tested. The the gene is mapped to chromosome 7q36. Cyclin-dependent kinase 5 is predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau. It is required for proper development of the mammalian central nervous system. To be activated, Cdk5 must associate with its regulatory subunit, p35(CDK5R1). It can phosphorylate DARPP32 at threonine-75, converting it into an inhibitor of PKA.
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