NSJ Bioreagents
SKU:RQ4295
CDC45 Antibody / PORC-PI-1
CDC45 Antibody / PORC-PI-1
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Cell division control protein 45 homolog was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Mouse, Rat |
| Predicted Species Reactivity | Human |
| Application | WB, ELISA |
| Application Details | Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml |
| Application Note | Optimal dilution of the CDC45 antibody should be determined by the researcher. |
| Localization | Cytoplasm, nucleus |
| Immunogen | A recombinant human protein corresponding to amino acids E166-A431 was used as the immunogen for the CDC45 antibody. |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| Purity | Antigen affinity purified |
| Storage | After reconstitution, the CDC45 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This CDC45 antibody is available for research use only. |
| Uniprot # | O75419 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/cdc45-antibody-porc-pi-1-rq4295 |
| Title | CDC45 Antibody / PORC-PI-1 |
| Description | Cell division control protein 45 homolog was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. |
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