• Flow cytometric analysis of NCI-H292 cells using CD8 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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NSJ Bioreagents

F43566-0.08ML

CD8 Antibody

CD8 Antibody

$205.00 USD
$205.00 USD
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The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, IHC, FACS, ELISA
Application Details Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 150-180 from the human protein was used as the immunogen for this CD8 antibody.
Purity Antigen affinity
Storage Aliquot the CD8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This CD8 antibody is available for research use only.
Uniprot # P01732
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cd8-antibody-f43566
Title CD8 Antibody
Description The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
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