• Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (ICO-115, red) and isotype control antibody (green). The PPI-negative cell population was gated for analysis.
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NSJ Bioreagents

V2065PE-100T

CD34 Antibody PE Conjugate

CD34 Antibody PE Conjugate

$559.00 USD
$559.00 USD
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This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.

Specifications

Family Primary antibody
Formulation 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format PE Conjugate
Clone ICO-115
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Species Reactivity Human and Rat. Other species not known.
Application FACS
Application Details Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence: 1:50-1:100
Application Note The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Localization Cell surface
Immunogen Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.
Purity Protein G affinity chromatography
Storage Store the CD34 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Limitation This antibody is available for research use only.
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cd34-antibody-pe-conjugate-ico-115-v2065pe
Title CD34 Antibody PE Conjugate
Description This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.
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