NSJ Bioreagents
SKU:V2994S-0.1ML
CD30 Antibody Cocktail
CD30 Antibody Cocktail
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Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | Bioreactor concentrate with 0.05% sodium azide |
| Format | Culture supernatant |
| Clone | Ber-H2 + CD30/412 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgGs |
| Species Reactivity | Human |
| Application | IHC-P |
| Application Details | Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT (1) |
| Application Note | Optimal dilution of the CD30 antibody cocktail should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Localization | Cell surface, cytoplasmic |
| Immunogen | A Co cell line established from a patient with Hodgkin's disease of T-cell lineage (Ber-H2) and human recombinant protein (CD30/412) were used as the immunogen for the CD30 antibody cocktail. |
| Purity | Unpurified high titer supernatant |
| Storage | Store the CD30 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide). |
| Limitation | This CD30 antibody cocktail is available for research use only. |
| Uniprot # | P28908 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/cd30-antibody-cocktail-ber-h2-cd30412-v2994s |
| Title | CD30 Antibody Cocktail |
| Description | Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45). |
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