NSJ Bioreagents
SKU:V2048PE-100T
CD20 Antibody PE Conjugate
CD20 Antibody PE Conjugate
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This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | PE Conjugate |
| Clone | B9E9 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a, kappa |
| Species Reactivity | Human. Other species not known. |
| Application | FACS, IF |
| Application Details | Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence (1:50-1:100) |
| Application Note | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. |
| Localization | Predominantly cell surface with some cytoplasmic |
| Immunogen | The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody. |
| Purity | Protein G affinity chromatography |
| Storage | Store the CD20 antibody at 2-8°C. The conjugate is light-sensitive, protect from light. |
| Limitation | This CD20 antibody is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/cd20-antibody-pe-conjugate-b9e9-v2048pe |
| Title | CD20 Antibody PE Conjugate |
| Description | This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression. |
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