• Western blot testing of human 1) MCF7, 2) K562 and 3) HEK293 cell lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms).
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NSJ Bioreagents

F54694-0.08ML

CCR4-NOT transcription complex subunit 4 Antibody / CNOT4

CCR4-NOT transcription complex subunit 4 Antibody / CNOT4

$205.00 USD
$205.00 USD
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CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, FACS, IHC-P
Application Details Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the CCR4-NOT transcription complex subunit 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic, nuclear
Immunogen Recombinant human protein was used as the immunogen for the CCR4-NOT transcription complex subunit 4 antibody.
Purity Antigen affinity purified
Storage Aliquot the CCR4-NOT transcription complex subunit 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This CCR4-NOT transcription complex subunit 4 antibody is available for research use only.
Uniprot # O95628
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/ccr4-not-transcription-complex-subunit-4-antibody-cnot4-f54694
Title CCR4-NOT transcription complex subunit 4 Antibody / CNOT4
Description CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
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