• Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO320, 4) HepG2, 5) placenta, 6) A549, 7) SKOV3, and 8) PANC-1 cell lysate with CBX3 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
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NSJ Bioreagents

RQ4075

CBX3 Antibody / HP1 gamma

CBX3 Antibody / HP1 gamma

$449.00 USD
$449.00 USD
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Chromobox protein homolog 3 is a protein that is encoded by the CBX3 gene in humans. At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, IF, FACS, ELISA
Application Details Western blot: 0.5-1ug/ml,IHC (FFPE & frozen): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the CBX3 antibody should be determined by the researcher.
Localization Nuclear
Immunogen A recombinant human partial protein corresponding to amino acids A2-Q183 was used as the immunogen for the CBX3 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Antigen affinity purified
Storage After reconstitution, the CBX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This CBX3 antibody is available for research use only.
Uniprot # Q13185
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cbx3-antibody-hp1-gamma-rq4075
Title CBX3 Antibody / HP1 gamma
Description Chromobox protein homolog 3 is a protein that is encoded by the CBX3 gene in humans. At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
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