• Western blot testing of human A549 cell lysate with Cathepsin L antibody. Expected molecular weight: 38-41 kDa with multiple smaller processed/active forms.
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NSJ Bioreagents

R30013

Cathepsin L Antibody

Cathepsin L Antibody

$449.00 USD
$449.00 USD
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Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.

Specifications

Family Primary antibody
Formulation 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Format Ascites
Clone 33/2
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Species Reactivity Human, Mouse, Rat
Application WB, IHC-F
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (Frozen): 4ug/ml-formalin/acetone fixed tissues
Application Note The stated application concentrations are suggested starting amounts. Titration of the Cathepsin L antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen Procathepsin L isolated from the human lung cancer cell line EPLC 32M1 was used as the immunogen for this Cathepsin L antibody.
Purity Ascites
Storage After reconstitution, the Cathepsin L antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Cathepsin L antibody is available for research use only.
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cathepsin-l-antibody-332-r30013
Title Cathepsin L Antibody
Description Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.
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