• Western blot testing of rat 1) liver, 2) testis, 3) stomach, 4) thymus and human 5) COLO-320, 6) HepG2, 7) 22RV1, 8) PANC-1 and 9) SGC-7901 lysate with Caspase 4 antibody at 0.5ug/ml. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit).
1 1

NSJ Bioreagents

RQ4340

Caspase 4 Antibody / CASP4 (large subunit)

Caspase 4 Antibody / CASP4 (large subunit)

$449.00 USD
$449.00 USD
Sale Sold out
Shipping calculated at checkout.
Size

Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, ELISA
Application Details Western blot: 0.5-1ug/ml, Direct ELISA: 0.1-0.5ug/ml (mouse recombinant protein)
Application Note Optimal dilution of the Caspase 4 antibody should be determined by the researcher.
Localization Cytoplasm
Immunogen A recombinant mouse protein corresponding to amino acids E108-T195 was used as the immunogen for the Caspase 4 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Antigen affinity purified
Storage After reconstitution, the Caspase 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Caspase 4 antibody is available for research use only.
Uniprot # P70343
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/caspase-4-antibody-casp4-large-subunit-rq4340
Title Caspase 4 Antibody / CASP4 (large subunit)
Description Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.
View full details