• Immunofluorescent staining of FFPE human U-2 OS cells with Calpain antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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NSJ Bioreagents

R31743

Calpain Antibody

Calpain Antibody

$449.00 USD
$449.00 USD
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Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, IF, FACS
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note The stated application concentrations are suggested starting amounts. Titration of the Calpain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen Human recombinant protein from the CAPN1 catalytic subunit (AA 396-555) was used as the immunogen for this Calpain antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Purity Antigen affinity
Storage After reconstitution, the Calpain antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Calpain antibody is available for research use only.
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/calpain-antibody-r31743
Title Calpain Antibody
Description Calpain 1 is also known as CAPN, CAPN1 and muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. The CPN1 gene encodes the catalytic subunit of the ubiquitous enzyme, Calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
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