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NSJ Bioreagents

SKU:F55047-0.08ML

Cadherin 20 Antibody / CDH20

Cadherin 20 Antibody / CDH20

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This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, FACS, IHC-P
Application Details Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note The stated application concentrations are suggested starting points. Titration of the Cadherin 20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 111-140 from the human protein was used as the immunogen for the Cadherin 20 antibody.
Purity Antigen affinity purified
Storage Aliquot the Cadherin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Cadherin 20 antibody is available for research use only.
Uniprot # Q9HBT6
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/cadherin-20-antibody-cdh20-f55047
Title Cadherin 20 Antibody / CDH20
Description This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.
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