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NSJ Bioreagents
SKU:F54735-0.08ML
Brachyury Antibody / Protein T / TBXT
Brachyury Antibody / Protein T / TBXT
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The protein encoded by the TBXT gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human |
| Application | WB, IF |
| Application Details | Western blot: 1:500-1:2000, Immunofluorescence: 1:25 |
| Application Note | The stated application concentrations are suggested starting points. Titration of the Brachyury antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Localization | Nuclear |
| Immunogen | A portion of amino acids 15-43 from the human protein was used as the immunogen for the Brachyury antibody. |
| Purity | Antigen affinity purified |
| Storage | Aliquot the Brachyury antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This Brachyury antibody is available for research use only. |
| Uniprot # | O15178 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/brachyury-antibody-protein-t-tbxt-f54735 |
| Title | Brachyury Antibody / Protein T / TBXT |
| Description | The protein encoded by the TBXT gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. |
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