• Western blot testing of BiP antibody and Lane 1: rat testis; 2: A549; 3: MCF-7; 4: HeLa cell lysate. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
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NSJ Bioreagents

R30913

BiP Antibody / GRP78 / HSPA5

BiP Antibody / GRP78 / HSPA5

$449.00 USD
$449.00 USD
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HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al.(2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al.(2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, IHC-F, ICC
Application Details Western blot: 0.5-1ug/ml,IHC (FFPE): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note The stated application concentrations are suggested starting points. Titration of the BiP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen An amino acid sequence from the C-terminus of human GRP78/BiP (EWLESHQDADIEDFK) was used as the immunogen for this BiP antibody (100% homologous in human, mouse and rat).
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity Antigen affinity
Storage After reconstitution, the BiP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This BiP antibody is available for research use only.
Uniprot # P11021
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/bip-antibody-grp78-hspa5-r30913
Title BiP Antibody / GRP78 / HSPA5
Description HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al.(2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al.(2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER.
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