• Western blot testing of 1) rat spleen, 2) rat stomach, 3) rat intestine, 4) human MCF7, 5) human A549 and 6) human SKOV lysate with BIK antibody. Expected molecular weight ~18 kDa but routinely observed at 18-22 kDa.
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NSJ Bioreagents

R32211

BIK Antibody / BCL2-interacting killer

BIK Antibody / BCL2-interacting killer

$449.00 USD
$449.00 USD
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Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, FACS
Application Details Western blot: 0.1-0.5ug/ml,IHC (FFPE): 0.5-1ug/ml,FACS: 1-3ug/10^6 cells
Application Note Optimal dilution of the BIK antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen Amino acids 1-123 of human BIK were used as the immunogen for the BIK antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Purity Antigen affinity
Storage After reconstitution, the BIK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This BIK antibody is available for research use only.
Uniprot # Q13323
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/bik-antibody-bcl2-interacting-killer-r32211
Title BIK Antibody / BCL2-interacting killer
Description Bcl-2-interacting killer is a protein that in humans is encoded by the BIK gene. This gene is mapped to 22q13.31. The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.
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