• IHC staining of FFPE human sarcoma with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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NSJ Bioreagents

RQ5390

BHC80 Antibody / PHF21A

BHC80 Antibody / PHF21A

$449.00 USD
$449.00 USD
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PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35/histone deacetylase complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, IF, FACS, Direct ELISA
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the BHC80 antibody should be determined by the researcher.
Immunogen Amino acids M1-Q97 from the human protein were used as the immunogen for the BHC80 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Affinity purified
Storage Store the BHC80 antibody at -20oC.
Limitation This BHC80 antibody is available for research use only.
Uniprot # Q96BD5
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/bhc80-antibody-phf21a-rq5390
Title BHC80 Antibody / PHF21A
Description PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35/histone deacetylase complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).
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