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NSJ Bioreagents
SKU:N1038-25UG
Beta Tubulin Antibody Loading Control
Beta Tubulin Antibody Loading Control
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There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat, Primate, Dog |
| Application | WB |
| Application Details | Western blot: 2-4ug/ml |
| Application Note | Provided assay concentrations are suggestions only, antibody titration may be required for optimal results. |
| Immunogen | A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody. |
| Buffer | 1X PBS, pH 7.4 |
| Purity | Protein A Affinity Chromatography |
| Storage | Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles. |
| Limitation | This beta Tubulin antibody loading control is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/beta-tubulin-antibody-loading-control-n1038 |
| Title | Beta Tubulin Antibody Loading Control |
| Description | There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate. |
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