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NSJ Bioreagents

SKU:F49314-0.08ML

Beta III Tubulin Antibody / TUBB3

Beta III Tubulin Antibody / TUBB3

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Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Predicted Species Reactivity Bovine, Chicken, Primate, Rat
Application WB, IHC, FACS, ELISA
Application Details Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note Titration of the beta III Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 36-63 from the human protein was used as the immunogen for this beta III Tubulin antibody.
Purity Antigen affinity
Storage Aliquot the beta III Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This beta III Tubulin antibody is available for research use only.
Uniprot # Q13509
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/beta-iii-tubulin-antibody-tubb3-f49314
Title Beta III Tubulin Antibody / TUBB3
Description Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
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