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NSJ Bioreagents

SKU:F54014-0.1ML

Beta Arrestin 1 Antibody

Beta Arrestin 1 Antibody

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Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation. The beta-arrestins target many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins) and recruiting the GPRCs to the adapter protein 2 complex 2 (AP-2) in clathrin-coated pits (CCPs). However, the extent of beta-arrestin involvement appears to vary significantly depending on the receptor, agonist and cell type. [UniProt]

Specifications

Family Primary antibody
Formulation In PBS with 50% glycerol and 0.03% ProClin 300
Format Purified
Clone 6A1-D7-C9
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Species Reactivity Human
Application WB
Application Details Western blot: 1:500
Application Note The stated application concentrations are suggested starting points. Titration of the Beta Arrestin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A human recombinant protein was used as the immunogen for this Beta Arrestin 1 antibody.
Purity Protein G affinity
Storage Store the Beta Arrestin 1 antibody at -20oC.
Limitation This Beta Arrestin 1 antibody is available for research use only.
Uniprot # P49407
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/beta-arrestin-1-antibody-6a1-d7-c9-f54014
Title Beta Arrestin 1 Antibody
Description Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation. The beta-arrestins target many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins) and recruiting the GPRCs to the adapter protein 2 complex 2 (AP-2) in clathrin-coated pits (CCPs). However, the extent of beta-arrestin involvement appears to vary significantly depending on the receptor, agonist and cell type. [UniProt]
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