NSJ Bioreagents
SKU:RQ4979
BCL9L Antibody
BCL9L Antibody
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B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB, IHC-P, IF, Direct ELISA |
| Application Details | Western blot: 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein) |
| Application Note | Optimal dilution of the BCL9L antibody should be determined by the researcher. |
| Immunogen | Amino acids E397-A666 from the human protein were used as the immunogen for the BCL9L antibody. |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| Purity | Affinity purified |
| Storage | Store the BCL9L antibody at -20oC. |
| Limitation | This BCL9L antibody is available for research use only. |
| Uniprot # | Q86UU0 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/bcl9l-antibody-rq4979 |
| Title | BCL9L Antibody |
| Description | B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514?517 and 970?973), and one poly-Ala segment (aa 900?903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391?1426. Over aa 1009?1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. |
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