• IHC testing of FFPE human breast cancer tissue with Bcl-W antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F54369-0.08ML

Bcl-W Antibody (BH3 domain specific)

Bcl-W Antibody (BH3 domain specific)

$205.00 USD
$205.00 USD
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Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, IHC-P
Application Details Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the Bcl-W antibody (BH3 Domain Specific) may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic
Immunogen A portion of amino acids 24-59 from the human protein was used as the immunogen for the Bcl-W antibody.
Purity SAS precipitation
Storage Aliquot the Bcl-W antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Bcl-W antibody is available for research use only.
Uniprot # Q92843
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/bcl-w-antibody-bh3-domain-specific-f54369
Title Bcl-W Antibody (BH3 domain specific)
Description Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
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