• Western blot testing of human HeLa cell lysate with PD-L1 / B7-H1 antibody at 0.5ug/ml. Expected molecular weight ~34/40-70 kDa (unmodified/glycosylated), observed here at ~62 kDa.
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NSJ Bioreagents

R32579

B7-H1 Antibody / PD-L1

B7-H1 Antibody / PD-L1

$449.00 USD
$449.00 USD
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B7 Homolog 1 (B7-H1), also known as CD274 or PD-L1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. B7-H1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of B7-H1 on tumor MDCs downregulates T-cell immunity and that B7-H1 blockade may represent an approach for cancer immunotherapy. Additionally, B7-H1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human
Application WB
Application Details Western blot: 0.5-1ug/ml
Application Note Optimal dilution of the B7-H1 antibody should be determined by the researcher.
Localization Cytoplasmic, membranous
Immunogen Amino acids 41-69 (KFPVEKQLDLAALIVYWEMEDKNIIQFVH-human) were used as the immunogen for the B7-H1 antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Purity Antigen affinity
Storage After reconstitution, the B7-H1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This B7-H1 antibody is available for research use only.
Gene ID # 29126.0
Uniprot # Q9NZQ7
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/b7-h1-antibody-pd-l1-r32579
Title B7-H1 Antibody / PD-L1
Description B7 Homolog 1 (B7-H1), also known as CD274 or PD-L1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. B7-H1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of B7-H1 on tumor MDCs downregulates T-cell immunity and that B7-H1 blockade may represent an approach for cancer immunotherapy. Additionally, B7-H1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection.
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