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NSJ Bioreagents
SKU:R30946
B Raf Antibody (BRAF)
B Raf Antibody (BRAF)
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BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf, also known as RAFB1. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts. B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB |
| Application Details | Western blot: 0.5-1ug/ml |
| Application Note | The stated application concentrations are suggested starting points. Titration of the B Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | Amino acids 344-362 (QPFRPADEDHRNQFGQRDR) were used as the immunogen for this B Raf antibody (100% homologous in human, mouse and rat). |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal |
| Purity | Antigen affinity |
| Storage | After reconstitution, the B Raf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This B Raf antibody is available for research use only. |
| Uniprot # | P15056 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/b-raf-antibody-braf-r30946 |
| Title | B Raf Antibody (BRAF) |
| Description | BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a serine/threonine-protein kinase called B Raf, also known as RAFB1. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts. B Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. |
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