• Western blot testing of 1) human Caco-2, 2) mouse kidney and 3) rat liver lysate with ATP7B antibody. Expected molecular weight: 140-157 kDa.
1 1

NSJ Bioreagents

F54249-0.08ML

ATP7B Antibody

ATP7B Antibody

$205.00 USD
$205.00 USD
Sale Sold out
Shipping calculated at checkout.
Size

ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, IHC-P, FACS, IF
Application Details Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:25-1:50,Immunofluorescence: 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the ATP7B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 1361-1391 from the human protein were used as the immunogen for the ATP7B antibody.
Purity Protein A purified
Storage Aliquot the ATP7B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This ATP7B antibody is available for research use only.
Uniprot # P35670
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/atp7b-antibody-f54249
Title ATP7B Antibody
Description ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
View full details