• IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F54457-0.08ML

ATP5O Antibody

ATP5O Antibody

$205.00 USD
$205.00 USD
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ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, FACS, IF, IHC-P
Application Details Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the ATP5O antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic
Immunogen A portion of amino acids 38-64 from the human protein was used as the immunogen for the ATP5O antibody.
Purity Antigen affinity purified
Storage Aliquot the ATP5O antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This ATP5O antibody is available for research use only.
Uniprot # P48047
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/atp5o-antibody-f54457
Title ATP5O Antibody
Description ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
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