NSJ Bioreagents
SKU:F54243-0.08ML
ATP5B Antibody
ATP5B Antibody
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ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human |
| Application | WB, IHC-P, FACS, IF |
| Application Details | Western blot: 1:1000,Immunohistochemistry (FFPE): 1:25-1:100,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25 |
| Application Note | The stated application concentrations are suggested starting points. Titration of the ATP5B antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Localization | Cytoplasmic |
| Immunogen | A portion of amino acids 135-163 from the human protein were used as the immunogen for the ATP5B antibody. |
| Purity | Antigen affinity purified |
| Storage | Aliquot the ATP5B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This ATP5B antibody is available for research use only. |
| Uniprot # | P06576 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/atp5b-antibody-f54243 |
| Title | ATP5B Antibody |
| Description | ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core. |
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