NSJ Bioreagents
SKU:RQ7217
ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha
ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha
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ATP synthase F1 subunit alpha, mitochondrial, also called ATP synthase subunit alpha and ATP5A1, is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB, IHC-P, IF, FACS, Direct ELISA |
| Application Details | Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml |
| Application Note | Optimal dilution of the ATP5A1 antibody should be determined by the researcher. |
| Immunogen | Recombinant human protein (amino acids R12-Q520) was used as the immunogen for the ATP5A1 antibody. |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| Purity | Antigen affinity purified |
| Storage | After reconstitution, the ATP5A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This ATP5A1 antibody is available for research use only. |
| Uniprot # | P25705 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/atp5a1-antibody-atp5f1a-atp-synthase-subunit-alpha-rq7217 |
| Title | ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha |
| Description | ATP synthase F1 subunit alpha, mitochondrial, also called ATP synthase subunit alpha and ATP5A1, is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. |
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