• Western blot testing of 1) rat stomach, 2) mouse stomach and 3) human SGC-7901 (human gastric cancer) lysate with ATP4B antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa (core peptide), ~52 kDa (beta subunit precursor), 60-80 kDa (glycosylated form).
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NSJ Bioreagents

R32734

ATP4B Antibody / Gastric hydrogen potassium ATPase beta

ATP4B Antibody / Gastric hydrogen potassium ATPase beta

$449.00 USD
$449.00 USD
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This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB
Application Details Western blot: 0.5-1ug/ml
Application Note Optimal dilution of the ATP4B antibody should be determined by the researcher.
Immunogen Amino acids 1-28 (MAALQEKKTCGQRMEEFQRYCWNPDTGQ) from the human protein were used as the immunogen for the ATP4B antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Purity Antigen affinity
Storage After reconstitution, the ATP4B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This ATP4B antibody is available for research use only.
Uniprot # P51164
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/atp4b-antibody-gastric-hydrogen-potassium-atpase-beta-r32734
Title ATP4B Antibody / Gastric hydrogen potassium ATPase beta
Description This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.
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