NSJ Bioreagents
SKU:F54717-0.08ML
ATG4D Antibody
ATG4D Antibody
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Species Reactivity | Human, Mouse |
| Application | WB, IHC-P |
| Application Details | Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25 |
| Application Note | The stated application concentrations are suggested starting points. Titration of the ATG4D antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Localization | Cytoplasmic |
| Immunogen | A portion of amino acids 220-249 from the human protein was used as the immunogen for the ATG4D antibody. |
| Purity | Purified |
| Storage | Aliquot the ATG4D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This ATG4D antibody is available for research use only. |
| Uniprot # | Q86TL0 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/atg4d-antibody-f54717 |
| Title | ATG4D Antibody |
| Description | Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. |
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